It's a method to detect specific DNA sequence or
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作者:fool (等级:11 - 出神入化,发帖:5183) 发表:2003-04-06 19:02:15  楼主  关注此帖评分:
Help for Genes &society!!!Who can explain the Southern blot for me? can not understand what the text book explains. Thanks a lot!!!
It's a method to detect specific DNA sequence or
gene expression. YOu use gel electrophoresis to separate the denatured DNA sequence. Transfer the DNA onto a cellulose paper for probing. Then you use a probe that can specifically bind to a sequence(or gene) you want to find. Usually radioactive labelled or chemiluminescence labelled for signal detection later.

Signal detected---> means there is gene you want to find in the DNA mixture applied to gel in the beginning.
Usually southern blotting is used for checking gene expression.
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