Help for Genes &society!!!
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作者:Ifaint (等级:0 - 我是小白,发帖:55) 发表:2003-04-06 17:55:03  楼主  关注此帖
Help for Genes &society!!!
Who can explain the Southern blot for me? can not understand what the text book explains. Thanks a lot!!!
Put your OWN COOL signature here!
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作者:Ifaint (等级:0 - 我是小白,发帖:55) 发表:2003-04-06 21:10:19  2楼
hehe, why di u change ur id?ashamed? hahaha.....
I didn't change my ID
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作者:Ifaint (等级:0 - 我是小白,发帖:55) 发表:2003-04-06 21:10:28  3楼
It's a method to detect specific DNA sequence orgene expression. YOu use gel electrophoresis to separate the denatured DNA sequence. Transfer the DNA onto a cellulose paper for probing. Then you use a probe that can specifically bind to a sequence(or gene) you want to find. Usually radioactive labelled or chemiluminescence labelled for signal detection later. Signal detected---> means there is gene you want to find in the DNA mixture applied to gel in the beginning. Usually southern blotting is used for checking gene expression.
Thanks a lot
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